Figure 3
From: FAT4 functions as a tumour suppressor in gastric cancer by modulating Wnt/β-catenin signalling
Effect of FAT4 knockdown on Wnt/ β -catenin signalling. (A) Altered nuclear translocation of β-catenin in response to FAT4 knockdown. Nuclear and cytosolic extracts of BGC-823 and HGC-27 cells were analysed by western blotting with Lamin A and β-actin as a loading control. (B) Cells were transfected with TOPflash or FOPflash and Renilla pRL-TK plasmids and subjected to dual-luciferase assays 48 h after transfection. Reporter activity was normalised to Renilla luciferase activity. (C) Western blot analysis of Wnt target gene expression in response to FAT4 knockdown in BGC-823 and HGC-27 cells. β-Actin was used as the loading control. **P<0.01 by Student’s t-test.
