Figure 6
From: FAT4 functions as a tumour suppressor in gastric cancer by modulating Wnt/β-catenin signalling
Effect of FAT4 knockdown on tumorigenesis and metastasis in vivo. (A) Photographs of excised tumours and tumour growth curves in mice transplanted with BGC-823/Scramble or BGC-823/FAT4 shRNA cells (FAT4 shRNA1) (N=5 per group). (B) After 32 days, the mice were killed and tumours were weighed. (C) IHC staining for FAT4 and Ki-67 in sections of transplanted tumours. (D) BGC-823/Scramble and BGC-823/FAT4 shRNA cells (6 × 106 cells per mouse) were injected into the tail vein of two groups of nude mice. Four weeks post injection, the mice were killed and the lungs and livers were removed and paraffin embedded. Arrows indicate surface metastatic nodules. (E) Number of visible surface metastatic lesions on the lungs and livers of individual mice (N=5 per group) receiving an orthotopic injection of the indicated cells. (F) Representative images of haematoxylin and eosin-stained lung and liver sections of mice injected with the indicated cells. *P<0.05, **P<0.01 by Student’s t-test.
