Figure 2

Surface phenotypes and T-cell stimulation capacity of TAA-pulsed mDCs in the preclinical study, which were performed as described in the Supplementary Method . (A) Representative FACS data (left) and statistical analysis (right) of the surface phenotype of imDCs and TAA-pulsed mDCs. (B) CD8⁺ T-cell population (left) and the levels of IFN-γ and IL-4 (right) were assessed from the co-culture of autologous T cells with TAA-pulsed or Ag-unpulsed mDCs at a 10 : 1 ratio in the presence of IL-7 (Peprotech). Activated T cells were restimulated three times every 7–8 days with a same set of TAA-pulsed DCs at the same ratio as described in the Supplementary Method. (C) From the activated T cells, the CTL was assessed with MHC-matched HepG2 target cells at different effector/target (E/T) ratios as described in the Supplementary Method. Data are presented as mean±s.d. of nine samples pooled from three individual experiments. *P<0.05 and **P<0.01 compared with control CTLs prepared by stimulation of T cells with Ag-unpulsed mDCs, Student’s t-test.