Figure 3
Immunological analysis after DC vaccination. (A) Antigen-specific lymphocyte proliferation assay was performed during and after DC vaccinations using the autologous PBMCs obtained from 12 patients (upper), and the results were further analysed in recurrence-free patients (lower left) and recurrent patients (lower right). The proliferation was determined by 3H-thymidine incorporation (dpm) using a liquid scintillation counter. Data are presented as mean±s.e., *P<0.05. (B) ELISPOT assay was performed with the PBMCs obtained from each patient at the indicated time points after the start of DC vaccination. The results from all (12) patients (upper), the nine recurrence-free patients (lower left) and the three recurrent patients (lower right) are presented as mean±s.e.
