Figure 3

GBM cells demonstrate a higher antioxidant activity against H₂O₂. (A) Both U373MG cells and HeLa cells were treated with increasing concentrations of H₂O₂ (0–2 mM). After 48 h cells were analysed using the Alamar Blue assay. All experiments were repeated at least three times. Statistical analysis was carried out using non-linear regression analyses. (B) Both U373MG cells and HeLa cells were preloaded for 1 h with 10 μ M H₂DCFDA. H₂DCFDA was removed and a dose-response analysis was performed using H₂O₂. The relative increase in fluroescence was determined 1 h after the addition of H₂O₂. Statistical analyses were carried out using non-linear regression analyses. All experiments were repeated minimum in triplicate. (C) In situ verification of ROS production in both U373MG cells and HeLa cells, which was measured 1 h after the addition of H₂O₂ by confocal microscopy using both 10 μ M H₂DCFDA and 2 μ M MitoSOX red, is shown. A full colour version of this figure is available at the British Journal of Cancer journal online.