Figure 5

Synergy observed between TMZ and NTAP. (A) The effects of multiple NTAP exposures were determined in both HeLa and U373MG. Cells were exposed three times within a 10-h period with a minimum 4 h between treatments. Forty-eight hours later, cells were then analysed using the Alamar blue assay. Results are shown as the mean±s.e.m. (n= minimum 24). Statistical analysis was carried out using one-way ANOVA with Tukey’s multiple comparison post-test (*P<0.05 against untreated control). (B) Following NTAP treatment, cells were treated with low concentrations of TMZ. Cells were incubated for 6 days and analysed by Alamar blue assay. Data shown were normalised to the untreated control and are shown as the % mean±s.e.m. (n=minimum 20). Statistical analysis was carried out using two-way ANOVA with Bonferroni post test (*P<0.001). All experiments were repeated at least three times.