Figure 2

Hypoxic conditions induce high levels of HK2 expression in neuroblastoma metastatic variants. Local and metastatic neuroblastoma variants were treated with 0, 25 and 50 μ M Deferoxamine (A) or incubated in a hypoxia chamber (1% oxygen) (B). Following treatment, the expression level of HIF-1α and HK2 was measured by western blot analysis. Shown are the results of one representative blot out of four independent experiments.