Figure 2
From: p63 is required beside p53 for PERP-mediated apoptosis in uveal melanoma
p63 is significantly down-regulated in UM cell lines. (A) Representative western blot showing relative protein levels of endogenous p63 protein in UM cell lysates. Skin cell lysate served as a positive control for p63 antibody. (B) mRNA was extracted from non-transfected (NT) OCM-1 as well as from A431 cells and p63-tGFP-transfected OCM-1 cells used as positive controls for p63 expression. Quantitative PCR was performed to assess mRNA levels by analysing the amplification plots and dissociation curves for each of the three types of cells. NTC, non-template control.
