Figure 3
From: p63 is required beside p53 for PERP-mediated apoptosis in uveal melanoma
Effect of p63 expression on OCM-1 cell viability. (A) OCM-1 cells were transiently transfected with p63-tGFP and the number of viable and nonviable cells in 10 different fields per dish (total number of fields analysed=30) was counted between 24 and 48 h after transfection using Annexin- V and PI as indicators of apoptosis. The mean percentage of transfected (or non-transfected) cells dead in the transfected or NT cell population with standard deviation from three independent transfections is shown (Students’s t-test, ***P⩽0.0001) compared with GFP-only transfected cells). (B) p63-tGFP transiently transfected OCM-1 cells were monitored through time-lapse confocal imaging for 24 h starting 24 h post-transfection, with images captured every 10 min. p63-tGFP-transfected cells showing nuclear localisation of p63 undergoing apoptosis are shown over time alongside non-transfected cells within the same cell population. (C) OCM-1 cells expressing p63-tGFP showing positive Annexin-V binding (blue) indicate early apoptosis, whereas the PI staining (red) highlights the cells that have lost membrane integrity.
