Figure 4
From: p63 is required beside p53 for PERP-mediated apoptosis in uveal melanoma
Increased levels of p63 expression lead to the upregulation of PERP mRNA.Total RNA was extracted from OCM-1 cells transiently transfected with GFP-only or p63-tGFP at the indicated times post-transfection (PT). p63 and PERP mRNA levels were determined by qPCR and were normalised to the endogenous level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The mean of three independent experiments along with s.d. is presented. Significance was evaluated using Student’s t-test, *P⩽0.04, compared with GFP-only-transfected cells.
