Figure 5
From: p63 is required beside p53 for PERP-mediated apoptosis in uveal melanoma
Elevated p63 expression leads to increase in endogenous PERP and p53 protein levels in OCM-1 cells. Cell lysates were prepared at 16, 24, 48 and 72 h post-transfection (PT) with p63-tGFP and analysed by western blotting alongside NT and GFP-only transfected cells that served as controls. A431 cell lysates served as a positive control for p63, p53 and PERP antibodies. p63, PERP and p53 proteins were detected with appropriate antibodies and their relative levels were quantified by densitometry and normalised to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The mean of three independent experiments along with s.d. is presented. Significance was evaluated with paired t-test, compared with GFP-only transfected cells. *P⩽0.03 (PERP) and *P⩽0.02 p53.
