Table 1 Gene-specific primer sets for real-time qPCR
From: p63 is required beside p53 for PERP-mediated apoptosis in uveal melanoma
Gene | Primer sequence | Annealing temperature (°C) |
|---|---|---|
PERP | 5′-CCAGATGCTTGTCTTCCTGAGAG-3′ 5′-AGTGACAGCAGGGTTGGCATGA-3′ | 65 |
p63 | 5′-CAGGAAGACAGAGTGTGCTGGT-3′ 5′-AATTGGACGGCGGTTCATCCCT-3′ | 65 |
GAPDH | 5′-AACAGCCTCAAGATCATCAG-3′ 5′-TGAGTCCTTCCACGATACC-3′ | 60 |
