Figure 2
Flow cytometric detection of cancer cells by staining for the tumour-associated cell surface markers CA9 and Cadherin 6, and the intracellular marker CK18. Cultured cancer cells were spiked to PBMCs at a concentration of 1%, and three-fold stepwise dilution series were prepared, resulting in concentrations of 1%, 0.33%, and 0.11%. Samples of PBMCs alone were used to detect background staining. The percentages show the amount of positive events for staining of the renal cancer cell line RCC4, visualised by plotting each marker (x axis) vs SSC properties (y axis) and gated based on isotype control (A). The same experiment was carried out by analysing the additional cell line CAKI6 (B) and ACHN3 respectively (C) respectively.
