Figure 1

Alkaline phosphatase is expressed by prostate cancer cells and increased in metastatic prostate cancer. (A) ALPL mRNA expression (normalised with GAPDH (glyceraldehyde 3-phosphate dehydrogenase) expression) was measured using qRT–PCR in a panel of prostate cancer cell lines, the breast cancer cell line MCF7 and the bone marrow stromal cell line HS5. Bars for metastatic cell lines (ARCaPM, C4-2B and PC3) are shaded in black. (B) ALPL mRNA expression (normalised with GAPDH expression) was measured using qRT–PCR and (C) alkaline phosphatase activity (normalised with total protein) was measured using a quantitative fluorimetric assay, in ARCaPE and ARCaPM cells. (D) ARCaPE, ARCaPM and 2T3 cells were stained for alkaline phosphatase activity. (E) The Tomlins et al. data set (Tomlins et al, 2007) was analysed for ALPL mRNA expression in benign prostatic hyperplasia, localised prostate cancer and metastatic prostate cancer samples. (**P<0.01, ***P<0.001).