Figure 4
Snail regulates expression of alkaline phosphatase in vitro and in vivo. In vitro, Snail expression was inhibited by transfecting siRNA in ARCaPM cells and (A) knockdown was confirmed using qRT–PCR. (B) Change in morphology after Snail knockdown was documented using phase-contrast microscopy. (C) Alkaline phosphatase activity was measured in siRNA-transfected ARCaPM cells using a quantitative fluorimetric assay. The correlation of (D) Snail mRNA or (E) CDH1 (E-cadherin) mRNA with ALPL mRNA expression was calculated in prostate cancer samples from the Taylor et al. data set (Taylor et al, 2010). In each case, the samples were also stratified into ALPL-high or ALPL-low samples (with median ALPL expression as the cutoff) and Snail or E-cadherin expression were plotted, respectively (NSP>0.05, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001).
