Figure 3

Downregulation of WWP1 expression by miR-452 in PC3 and DU145 cells. (A) WWP1 mRNA expression 72 h after transfection with miR-452. GUSB was used as an internal control. (B) WWP1 protein expression 72 h after transfection with miR-452. GAPDH was used as a loading control. (C) miR-452 binding sites in WWP1 mRNA. Luciferase reporter assays were carried out using a vector encoding the putative miR-452 target site in the WWP1 3′-UTR (position 150–156) for wild-type and deletion constructs. *P<0.0001. The bars indicate s.d.’s.