Figure 4

Effects of WWP1 knockdown on cell proliferation, migration, and invasion in PCa cells and expression of WWP1 in clinical PCa specimens. (A) Cell proliferation was determined by XTT assays. (B) Cell migration activity was determined using uncoated Transwell polycarbonate membrane filters. (C) Cell invasion activity was determined by Matrigel invasion assays. (D) Representative image of IHC of WWP1 in the tissue microarray. (E) WWP1 was upregulated in PCa compared with PIN and normal tissue in the tissue microarray. *P<0.0001. The bars indicate s.d.’s.