Figure 2

Overexpression of PICK1 inhibits bone metastasis of PC-3 cells in vivo. (A) Representative BLI signal of bone metastasis in a mouse from the indicated groups of mice at 3 min and 8 weeks. (B) Representative radiographic images of bone metastases in the indicated mice (arrows indicate osteolytic lesions). (C) Representative H&E-stained sections of tibias from the indicated mouse (T, tumour; N, the adjacent non-tumour tissues). (D) The sum of bone metastasis scores for each mouse among tumour-bearing mice inoculated cells carrying the vector (n=8) or a PICK1-overexpression plasmid (n=9). *P<0.05. (E) Quantification of BLI signals in the vector and PICK1-overexpression groups at 6, 7 and 8 weeks. *P<0.05. (F) Kaplan–Meyer analysis of mouse survival for the vector and PICK1-overexpression groups. (G) Kaplan–Meyer analysis of mouse bone metastasis-free survival for the vector and PICK1-overexpression groups.