Figure 5
Platelet releasates enhance tumour growth in vivo. MDA-MB-231 breast cancer cells in Matrigel (106 cells in 250 μl) were subcutaneously implanted without or with 10% (final concentration) PAR1-PR or PAR4-PR in female nude mice. After 4 weeks, tumours were surgically removed, weighted, and sectioned for HE staining. (A) Tumour growth ratios were calculated using the following formula: tumour growth ratio=(tumour weight with vehicle (or PAR1-PR or PAR4-PR))/tumour weight with vehicle. Mean±s.e.m. from eight tumour pairs are plotted. *P<0.05 vs vehicle, †P<0.05 vs PAR1-PR. Representative images of MDA-MB-231 cell tumour sections with vehicle (B), PAR1-PR (C), and PAR4-PR (D) are shown. Black asterisks indicate cancer tissues, and white arrows indicate vessels in the tumour plugs. A full colour version of this figure is available at the British Journal of Cancer journal online.
