Figure 2
From: MDM2 promotes epithelial–mesenchymal transition and metastasis of ovarian cancer SKOV3 cells
MDM2 induced EMT in ovarian cancer cells. (A) Overexpression of MDM2 caused morphologic changes to a mesenchymal phenotype in SKOV3 cells. Representative photos were as indicated, scale bar=50 μm. (B) Transfection of MDM2 induced downregulation of E-cadherin protein levels, which is determined by western Blot. (C) The TGF-β (5 ng ml−1) treatment stimulated morphologic changes to mesenchymal phenotype, which is subsequently rescued by MDM2 depletion, scale bar=50 μm. (D) Knockdown of MDM2 increased E-cadherin protein expression, and abrogated the loss of E-cadherin upon TGF-β treatment. (E) The mRNA levels of E-cadherin expression were monitored by qRT-PCR assay. The MDM2 exogenous transfection resisted the E-cadherin transcription, whereas MDM2 siRNA upregulated the E-cadherin mRNA levels. (F) The MDM2 knockdown reversed the decline of E-cadherin mRNA triggered by TGF-β. Data were presented as means ± s.d. from three independent experiments. *P<0.05; ***P<0.001, as evaluated using Student's t-test.
