Figure 3
From: MDM2 promotes epithelial–mesenchymal transition and metastasis of ovarian cancer SKOV3 cells
MDM2 regulated the TGF- β -induced Snail/Slug activation in ovarian cancer cells. (A) MDM2 plasmid was introduced to SKOV3 cells and raised the protein expression of Snail and Slug. (B) The qRT-PCR analyses revealed that the mRNA levels of Snail and Slug were upregulated by MDM2 overexpression. (C) The MDM2 siRNA abrogated the induction of Snail and Slug protein by TGF-β (5 ng ml−1). (D) MDM2 knockdown significantly repressed the mRNA levels of Snail and Slug increased by TGF-β. (E) SKOV3 cells treated with scramble or MDM2 siRNA were transfected with plasmids encoding SBE-luciferase and pRL-IRES Renilla in the presence or absence of TGF-β (5 ng ml−1) for 24 h. MDM2 depletion depressed the TGF-β-induced-SBE activity, which was measured by Luciferase assay. Data were presented as means ± s.d. from three independent experiments. *P<0.05; ***P<0.001, as evaluated using Student's t-test.
