Figure 2

Enhanced and directly correlated PD1 and CD3 expression in the tumoural region in NSCLC, especially in ADC. Immunostainings are represented at × 40 magnifications (and in the upper left square a reduction is shown at × 20) by using the computer program Case Viewer. (A and B) Paraffin-embedded lung tissue slices of the pathological verified CTR and TU from patients who suffered from ADC were immunohistochemically stained with αIL-10 and αPD1 antibodies. ADC CTR n=12; ADC TU n=9 and SCC CTR n=9; SCC TU n=9, respectively. (C) Quantification of PD1⁺ cells in NSCLC patients. ADC CTR n=10; ADC TU n=10; SCC CTR n=9; SCC TU n=9. (D) Correlation between the area fraction of PD1⁺ and CD3⁺ cells in TU of patients who suffered from ADC. Coincident pairs ADC TU n=8. (E) Correlation between PD1 mRNA level and PDL1 mRNA level in the TU of patients with SCC. Coincident pairs n=14. (F) Correlation between PD1 mRNA level and PDL1 mRNA level in the TU of patients with NSCLC. Coincident pairs NSCLC TU n=27. Bar chart is shown as mean values±s.e.m. using Student's t-test *P<0.05, **P<0.01 and ***P<0.001.