Figure 2

AR-positive RCC cell lines have an intracrine androgen biosynthesis pathway.(A) ELISA was carried out for the intracellular testosterone (T) and dihydrotestosterone levels in the listed human RCC cell lines. Caki2 and Hs891.T are AR-positive. The human prostate cancer cell line, LNCaP, was used as the positive control. Higher levels of T and DHT were found in the AR-positive human RCC cell lines. (B) RT-PCR analysis for the expression patterns of key enzymes required for the intracellular androgen biosynthesis was carried out in each cell line. Again, LNCaP was used as the positive control. With the exception of CYP11A1, all of the key enzymes were detected in only the AR-positive human RCC cell lines, Caki2 and HS891.T. *statistically significant (with respect to ACHN).