Figure 3
Direct regulation of ITGA3 by miR-199a/b-5p and miR-199a/b-3p. (A) The expression of ITGA3 mRNA was significantly repressed in these miRNAs transfectants in comparison with that in mock or miR-control transfectants. GUSB was used as an internal control. *P=0.0002; **P<0.0001. (B) The expression of ITGA3 protein was markedly repressed in these miRNAs transfectants in comparison with that in mock or miR-control transfectants. β-Actin was used as a loading control. (C) Dual-luciferase reporter assays using vectors encoding putative miRNA target sites for wild-type or deleted regions. Normalised data were calculated as ratios of Renilla/firefly luciferase activities. The luminescence intensity was significantly reduced by co-transfection with miR-199a/b-5p or miR-199a/b-3p and the vector carrying the wild-type sequences at positions 395–401 and 439–452 in the ITGA3 3′-UTR. *P<0.0001, **P=0.0001.
