Figure 1
Combinational cytotoxicity of dasatinib and SN38.(A) Combination treatment of dasatinib and SN38 induced cytotoxicity in HCC cell lines, including BEL-7402 and HepG-2. And 4 × 103 cells per well were cultured in 96-well plates and exposed to different concentrations of dasatinib and/or SN38 for 48 h. Survival fractions were calculated and shown as mean±s.d. from three independent experiments. (B) CI values at different ratio concentrations of dasatinib and SN38 were calculated by the proliferation inhibition rates. (C) The inhibition of cell proliferation was observed by optical microscopy after combination treatment for 48 h (magnification × 200). (D) Colony-formation assays of BEL-7402 and HepG-2 were performed. One thousand cells were plated in a six-well plate and incubated with dasatinib and/or SN38 for 10 days and then photographed. (E) The colony-formation ability of each group was shown in bar chart and described as mean±s.d. from three independent experiments. *Compared with the combination group, P<0.05.
