Table 1 invasive scrotal cancers by country, year of/age at diagnosis, histological category, HPV DNA detection and genotyping by 4 assays, HPV histological localization by LCM, HPV16 E6*I mRNA detection, p16INK4a and p53 overexpression
From: Human papillomavirus 16 is an aetiological factor of scrotal cancer
Cases studied | ||||||
|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | |
Country of origin | Spain | Spain | Australia | Australia | Nigeria | Nigeria |
Year of diagnosis | 2003 | 2008 | 2002 | 2007 | 2007 | 2015 |
Age at diagnosis | 71 | 62 | 59 | 46 | 42 | 66 |
Histological diagnosis | Usual SCC | Usual SCC | Warty SCC | Usual SCC | Basaloid SCC | Basaloid SCC |
HPV DNA and RNA analysis | ||||||
SPF10−DEIA-LiPA25 | HPV negative | HPV negative | HPV16 | HPV negative | HPV 16 | HPV 16 |
MPTS123-PCR Luminex | HPV negative | HPV negative | HPV 16 | HPV negative | HPV 16 | HPV 16 |
Beta HPVs assay | HPV negative | HPV negative | HPV negative | HPV negative | HPV negative | HPV negative |
Cutaneous Wart-Associated HPVs | HPV negative | HPV negative | HPV negative | HPV negative | HPV negative | HPV negative |
HPV16 E6*I mRNA-Luminex | HPV negative | HPV negative | Positive | HPV negative | Positive | Positive |
DNA and RNA quality control | Positive | Positive | Positive | Positive | Positive | Positive |
Laser capture microdissection | ||||||
Localization of HPV | n.a. | n.a. | SCC | n.a. | SCC | SCC |
Immunohistochemistry analysis in primary cancer cells | ||||||
p16INK4a overexpression | <5% | 25% | >75% | <5% | >75% | >75% |
p53 overexpression | >75% | <25% | <25% | >75% | <25% | <25%a |
