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Cell Procurement

The CXCL12-3′A allele is associated with a higher mobilization yield of CD34 progenitors to the peripheral blood of healthy donors for allogeneic transplantation

Bone Marrow Transplantation volume 44, pages 273–278 (2009)Cite this article

Abstract

The interaction between CXCL12 and its receptor CXCR4 plays a crucial role in the homing and mobilization of haematopoietic progenitors. We investigated the putative association between a CXCL12 gene polymorphism, the G → A transition at position 801 in the 3′-untranslated region (3′UTR), and the yield of CD34+ progenitors in 65 healthy allogeneic transplant donors who received G-CSF. Importantly, in this setting, the analysis was not biased by background disease or chemotherapy. The 3′UTR CXCL12 G801A polymorphism was detected using a PCR-RFLP technique with the MspI restriction enzyme and the frequency of CD34+ progenitors was assessed by flow cytometry. The frequency as well as the number of CD34+ progenitor cells in the first leukapheresis product was significantly higher from donors with the CXCL12-3′A allele compared to GG homozygotes (P<0.05 in both cases), especially for subjects with the CXCL12-3′AA homozygous genotype (P<0.01 in both cases). Moreover, more leukaphereses were needed to obtain the required number of CD34+ progenitors for transplantation from CXCL12-3′GG homozygous donors compared to the CXCL12-3′A carriers (P=0.003). In conclusion, the CXCL12-3′A allele was associated with a higher yield of CD34+ cells from healthy donors of PBPC for allogeneic haematopoietic SCT.

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Acknowledgements

We especially thank Danuta Sosnowska from the Cytopheresis Unit of the Lower Silesian Center for Cellular Transplantation for her help in collecting the donors’ data.

This work was supported by grants from the Polish Ministry of Science and Higher Education (2 PO5B 085 28, N N401 008035 and N N402 193335) and the FP6 Grant AlloStem (LSHB-CT-2004-503319).

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Authors and Affiliations

  1. L Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland

    K Bogunia-Kubik, A Gieryng, D Dlubek & A Lange

  2. Lower Silesian Center for Cellular Transplantation and National Polish Bone Marrow Donor Registry, Wroclaw, Poland

    D Dlubek & A Lange

Authors
  1. K Bogunia-Kubik
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  2. A Gieryng
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  3. D Dlubek
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  4. A Lange
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Correspondence to K Bogunia-Kubik.

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Bogunia-Kubik, K., Gieryng, A., Dlubek, D. et al. The CXCL12-3′A allele is associated with a higher mobilization yield of CD34 progenitors to the peripheral blood of healthy donors for allogeneic transplantation. Bone Marrow Transplant 44, 273–278 (2009). https://doi.org/10.1038/bmt.2009.30

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