Figure 2
From: Superselective intra-arterial umbilical cord blood administration to BM in experimental animals
IHC staining of the femoral BM in the rabbit. Intravital microscopy in C57BL/6 mice. (a, b) Intravital microscopy of the cremaster muscle in a C57BL/6 mouse immediately after i.a. injection of 5 × 106 fluorescence-labeled human UCB cells into the iliac artery. (a) Cells entering the capillary bed by an arteriole. Blood flow direction is indicated by the arrow. (b) Cells leaving the capillary bed by a venule. Blood flow direction is indicated by the arrow. The slowly moving cells in the capillary bed are seen in both (a) and (b). In (c) photomicrograph showing immunostaining for HuNu (human nuclear antigen) positive cells in the femoral rabbit BM after injection of human UCB cells into the femoral nutrient artery. The magnification shows the immunohistochemically labeled cells. (d) Photomicrograph showing a section for the IHC from an i.v.-injected animal. Scale bars=20 μm.
