Figure 4
From: Comparison of Effects of Mechanical Stretching on Osteogenic Potential of ASCs and BMSCs
The mRNA level of BMP-2 (A) in ASCs increased by 3-fold after two hours of mechanical stretch (P<0.01) and 4-fold after six hours of mechanical stretch (P<0.01) compared to the unloaded control; the mRNA level of BMP-2 in BMSCs increased by 4-fold after 6 hours (P<0.01). When cells were mechanically stretched for 2 hours, BMP-2 mRNA in ASCs increased more significantly (P<0.05) than BMSCs. The mRNA level of Runx2 (B) in ASCs increased by 1.5-fold after 6 hours of mechanical loading (P<0.05) compared to the unloaded control; the mRNA level of Runx2 in BMSCs increased by 2-fold after 2 and 6 hours of mechanical loading (P<0.05). No significant differences of Runx2 mRNA expression were observed between ASCs and BMSCs. The mRNA level of Osx (C) of ASCs and BMSCs were both significantly enhanced (P<0.01). When cells were mechanically stretched for 2 hours, Osx mRNA in ASCs increased more significantly than BMSCs (P<0.05). Mechanical stretch promoted the OCN mRNA (D) expression of ASCs rather than OCN mRNA in BMSCs. The mRNA level of OCN in ASCs increased by 1-fold when loaded for 6 hours (P<0.05) compared to the unloaded control. However, mRNA level of OCN in BMSCs did not changed significantly.
