Figure 6
From: LRP6 in mesenchymal stem cells is required for bone formation during bone growth and bone remodeling
Bone marrow MSCs with LRP6 deletion had reduced rate of survival and diminished ability of proliferation and colony forming. (a and b) Representative immunofluorescence staining of nestin (red) in femur sections from male Lrp6+/+ and Lrp6−/− mice (a). Scale bars=100 µm. Quantitative analysis of the percentage of nestin+ cells per mm2 tissue area (b). A total of three femur sections from each mouse, and five mice per treatment group were analyzed. *P<0.001, vs. Lrp6+/+ group. (c) Colony-forming potential of bone marrow cells from 3-month-old male Lrp6+/+ , Lrp6+/− and Lrp6−/− mice. (d) Representative phase-contrast micrographs of bone marrow MSCs sorted from 3-month-old male Lrp6+/+ , Lrp6+/− and Lrp6−/− mice. (e) Population doubling time in days of MSCs infected with adenovirus containing GFP (control) or Cre between passage 1 (P1) and passage 2 (P2). n=5 for each treatment group; *P<0.001. (f) Representative immunofluorescence staining of BrdU (red) in MSCs infected with adenovirus containing GFP or Cre and labeled with BrdU. Nuclei were counterstained with DAPI (blue). (g) Quantitative analysis of the percentage of BrdU+ cells out of the total cells. Cells were counted from four random high power fields per slides. n=5 slides for each treatment group; *P<0.001. (h) Quantitative analysis of the percentage of dead cells out of the total MSCs infected with adenovirus containing GFP (control) or Cre. n=5 for each treatment group; *P<0.001. BrdU, 5-bromo-2′-deoxyuridine; DAPI, 4′,6-diamidino-2-phenylindole; GFP, green fluorescent protein.
