Figure 2
From: Rho/Rock signal transduction pathway is required for MSC tenogenic differentiation
MSC differentiation toward tenocytes on rope-like silk scaffold measured by gene and protein expression. (a) Relative mRNA expression levels of Scx, Tnmd, Nst, Oct4, Sox9, Col2a1, Runx2, and Alp in MSCs grown on silk-coated plates or silk scaffolds at 7 days after MSCs were seeded. (b) Immunocytochemistry staining (blue = DAPI, red = SCX or TNMD) and (c) western blot of SCX and TNMD in cells that were seeded on silk-coated plates and silk scaffolds. Data represent three independent experiments per assay. *P < 0.05 compared to MSCs grown on silk-coated plates. Scale bar = 50 µm.
