Table 1 PCR primers pairs
From: Rho/Rock signal transduction pathway is required for MSC tenogenic differentiation
Gene | Forward | Reverse |
Scx | 5′-AAACAGATCTGCACCTTCTG-3′ | 5′-TCAGATCAGGTCCAAAGTGG-3′ |
Tnmd | 5′-ATGACATGGAGCACACTTC-3′ | 5′-AAGTAGATGCCAGTGTATCC-3′ |
Col1a1 | 5′-TGACTGGAAGAGCGGAGAGT-3′ | 5′-GTTCGGGCTGATGTACCAGT-3′ |
Nfatc4 | 5′-ACCGGATTACTGGCAAGATG-3′ | 5′-CTGTCTCACCCTTCCGTAGC-3′ |
Nst | 5′-GTCTGATCTAGTACCAAAGG-3′ | 5′-CTTGCCACAGCAGCAGATTTTGC-3′ |
Oct4 | 5′-AGTGGGGCGGTTTTGAGTAA-3′ | 5′-TTCCAAAGAGAACGCCCAGG-3′ |
Sox9 | 5′-TCCCCGCAACAGATCTCCTA-3′ | 5′-AGGTGGAGTAGAGCCCTGAG-3′ |
Col2a1 | 5′-GCCAGGATGCCCGAAAATTAG-3′ | 5′-CGCACCCTTTTCTCCCTTGT-3′ |
Runx2 | 5′-CGTCGTCAGACCGAGAAGTG-3′ | 5′-GGATCTCGGGGCGACAGA-3′ |
Alp | 5′-TGGTCACAGCAGTTGGTAGC-3′ | 5′-ACCCTGAGATTCGTCCCTCG-3′ |
Gapdh | 5′-CTGCCAAGTATGATGACATC-3′ | 5′-TGTCATACCAGGAAATGAGC-3′ |
Hprt1 | 5′-GAAGTGTTGGATACAGGCCAGA-3′ | 5′-TTGCGCTCATCTTAGGCTTTG-3′ |
Pgk1 | 5′-GGGAACCAAGTCACTCATGGA-3′ | 5′-GGAGCTCTAGACTGGCACCG-3′ |
