Figure 5
From: Notch signaling controls chondrocyte hypertrophy via indirect regulation of Sox9
Notch regulation of terminal chondrocyte maturation is not likely to be mediated via Sox9. (a) Histological and molecular analysis of control, Prx1Cre;RBPjkf/f, Prx1Cre;RBPjkf/f; Sox9f/+ embryonic tibia sections at E18.5. ABH/OG staining (a1–a3). Markers of chondrocyte maturation – Indian Hedgehog (Ihh) (a4–a6), Collagen 10a1 (Col10a1) (a7–a9) and Matrix Metaloproteinase 13 (Mmp13) (a10–a12) were analyzed by in situ hybridization. (b) Immunohistochemistry for SOX9 in control (b1), Prx1Cre;RBPjkf/f (b2), and Prx1Cre;RBPjkf/f ;Sox9f/+ (b3) embryonic tibia sections at E18.5. Yellow double-headed arrows indicate the length of the SOX9 expressing hypertrophic chondrocyte domain. Red double-headed arrows indicate the total length of the hypertrophic zone.
