Figure 3
From: Ex vivo 3D osteocyte network construction with primary murine bone cells
Time-lapsed micrographs showing the morphological changes of cells migrated out of bone chips: (a) mostly round and floating cells with some attached cells forming short dendrite processes at Day 1, (b) attached cells forming longer dendrite processes at Day 4, (c) development of wide/spread cells (solid arrow) and elongated cells (dash arrow) at Day 7, (d) colony formation by wide/spread cells at Day 7, (e) domination of wide/spread cell colonies at Day 12, and (f) alizarin red staining showing significant mineral production at Day 40. Scale bar: 100 µm.
