Figure 3
From: A review of UHMWPE wear-induced osteolysis: the role for early detection of the immune response
Binding assay of NIRF probe cFLFLF-PEG-Cy7 to RAW264.7 cells stimulated by 1 μg·mL−1 lipopolysaccharide vs no treatment (NT).90 (a) Various concentrations of the probe were applied to the assay, including (from top to bottom) 1.0, 0.5, 0.1 and 0.05 μmol·L−1. Results indicate that fluorescent intensity strengthened with increasing doses of the probe, and that LPS was able to enhance binding affinity of the probe for cells. (b) A blocking test was performed with cFLFLF and a Scramble peptide (cLFFFL), revealing that the binding signal was blocked by cFLFLF but not controls.
