Figure 2
miR-23a/b inhibits the adipogenic differentiation of BMSCs. (a) qRT-PCR analysis of the relative levels of miR-23a/b in BMSCs induced to differentiate into adipocytes for 14 days. (b) The relative levels of miR-23a/b in BMSCs transfected with 10 μmol·L−1 agomiR-23a/b, antagomiR-23a/b or their NC were analyzed by qRT-PCR. (c and d) Representative images of Oil Red staining of lipid droplets (c), and the quantitative analysis of the number of Oil Red spots (d) in BMSCs induced to differentiate into adipocytes for 14 days. (e and f) The relative mRNA expression levels of adipogenic markers, Pparg (e) and Fabp4 (f), were measured by qRT-PCR in BMSCs induced to differentiate into adipocytes for 48 h. Scale bars: 120 μm. n=5 per group. Data are shown as the mean±s.d. *P<0.05, **P<0.01 (ANOVA). ANOVA, analysis of variance; BMSCs, bone marrow mesenchymal stem cells; NC, negative controls; qRT-PCR, quantitative reverse transcription PCR.
