Table 1 Primer sequences used for qRT-PCR detection for mRNAs

From: miR-23a/b regulates the balance between osteoblast and adipocyte differentiation in bone marrow mesenchymal stem cells

Gene

Acc. No

Primer sequence (5′ to 3′)

Pparg (mouse)

NM_001127330

F: GACCACTCGCATTCCTTT

  

R: CCACAGACTCGGCACTCA

Fabp4 (mouse)

NM_024406

F: AAATCACCGCAGACGACA

  

R: CACATTCCACCACCAGCT

Runx2 (mouse)

NM_001146038

F: ACTTCCTGTGCTCCGTGCTG

  

R: TCGTTGAACCTGGCTACTTGG

Osterix (mouse)

NM_130458

F: ACCAGGTCCAGGCAACAC

  

R: GCAAAGTCAGATGGGTAAGTAG

Tmem64 (mouse)

NM_181401

F: AGGAAGCGGCCTGAAGGT

  

R: GAAGGAAGAGCCACTGGGAT

β-actin (mouse)

NM_007393

F: CTGTCCCTGTATGCCTCTG

  

R: TGATGTCACGCACGATTT

  1. Acc. No, Genbank accession numbers; F, forward primer; mRNA, messenger RNA; R, reverse primer; qRT-PCR, quantitative reverse transcription PCR.
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