Figure 2
From: DNA N6-methyladenine demethylase ALKBH1 enhances osteogenic differentiation of human MSCs
Depletion of ALKBH1 inhibits osteogenic differentiation. (a) Real-time RT-PCR. n=3. ***P<0.001. (b) Western blot analysis. (c) Representative images of dot blot assay. (d) Representative image of alkaline phosphatase (ALP) staining. (e) Quantitative analyses of the ALP activity. n=5. **P<0.01. (f) Representative image of Alizarin red S (ARS) staining of MSCs. (g) Quantitative analyses of calcium mineralization. n=5. **P<0.01. (h–j) Real-time RT-PCR revealed reduced RUNX2, SP7, and BGLAP messenger RNA expression. n=3. *P<0.05, **P<0.01 and ***P<0.001.
