Table 1 Primers for quantitative RT-PCR and ChIP-qPCR
From: DNA N6-methyladenine demethylase ALKBH1 enhances osteogenic differentiation of human MSCs
Gene | Primers |
|---|---|
For RT-PCR | |
 ALKBH1 F | AGAAGCGACTAAACGGAGACC |
 ALKBH1 R | GGGAAAGGTGTGTAATGATCTGC |
 RUNX2 F | GCAAGGTTCAACGATCTGAG |
 RUNX2 R | GGAGGATTTGTGAAGACGGT |
 SP7 F | GAGGCAACTGGCTAGGTGG |
 SP7 R | CTGGATTAAGGGGAGCAAAGTC |
 BGLAP F | GGCGCTACCTGTATCAATGG |
 BGLAP R | GTGGTCAGCCAACTCGTCA |
 ATF4 F | ATGACCGAAATGAGCTTCCTG |
 ATF4 R | GCTGGAGAACCCATGAGGT |
 GAPDH F | TCATTGACCTCAACTACATG |
 GAPDH R | TCGCTCCTGGAAGATGGTGAT |
For ChIP | |
 ATF4 F | TGGCAGCAACAGCAACCATTA |
 ATF4 R | TGAGCCTCAGTTTCCTCATTTGTA |
