Figure 3 | Bone Research

Figure 3

From: Enhanced but hypofunctional osteoclastogenesis in an autosomal dominant osteopetrosis type II case carrying a c.1856C>T mutation in CLCN7

Figure 3

Enhanced but heterogeneous osteoclast (OC) formation in autosomal dominant osteopetrosis type II (ADO2). Peripheral blood mononuclear cells (PBMCs) were induced to differentiate into OCs in the presence of macrophage-colony stimulating factor (M-CSF) and RANKL for 7 days (a–c,e-g). (a–c) TRAP-positive multinuclear cells in the control. Note the heterogeneous OCs in the ADO2 cells (e-g). (e) Seemingly normal OCs. (f) Giant OC with large numbers of nuclei (arrow). (g) Abnormal TRAP-positive multinuclear cells (arrows). The ADO2 osteoclast nuclei were always arranged in circles. (d,h) PBMCs were cultured in the absence of M-CSF and RANKL for 7 days. TRAP-positive multinuclear cells spontaneously formed from ADO2 cells (red arrows) (h). No multinucleated osteoclast-like cells were observed in the control cells (d). (i) The number of seemly normal OC in the ADO2 culture was significantly lower than that in the control culture.

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