Figure 2

Determination of sCSF1 RNA expression levels in sCSF1 K/O mice. Isoform-specific RT-PCR of bone RNA demonstrates the absence of sCSF1 transcripts in sCSF1 K/O mice. Arrow indicates the expected amplicon, which is present in Wt but not in K/O animals. K/O, knockout; RT-PCR, reverse transcription PCR.