Figure 4
TNF-α inhibits osteogenesis in a miR-146a-dependent manner. (a) Osteogenic differentiation decreasesmiR-146a expression, and TNF-α dose dependently increases miR-146a expression. (b) Overexpression and knockdown efficiency of miR-146a. (c) BMSCs were treated as indicated, and osteogenesis was observed by Alizarin Red staining. GM, growth medium; OM, osteogenic medium with BMP2 (50 ng·mL−1). (d) BMSCs were treated as indicated, and osteogenesis was observed by western blot analysis of Runx2. (e–g) The expression of Runx2 (e), BSP (f) and ALP (g) at the RNA level was analyzed by qPCR. n≥3, *P<0.05.
