Table 1 Sequences of the primers or miRNA inhibitors/mimics used in the study

From: Dysregulation of the miR-146a-Smad4 axis impairs osteogenesis of bone mesenchymal stem cells under inflammation

Names

Forward primers (5ʹ-3ʹ)

Reverse primers (5ʹ-3ʹ)

Runx2

ACACCGTGTCAGCAAAGC

GCTCACGTCGCTCATCTTG

Alp

AACAACCTGACTGACCCTTCG

AATCCTGCCTCCTTCCACC

Bsp

GTCTTTAAGTACCGGCCACG

TGAAGAGTCACTGCCTCCCT

Smad4

TCACTATGAGCGGGTTGTCTC

TCCTTCAGTGGGTAAGGACG

Gapdh

CGTCCCGTAGACAAAATGGT

TTGATGGCAACAATCTCCAC

miR-146a

TGAGAACTGAATTCCATGGG

Universal primer provided

U6

CTCGCTTCGGCAGCACA

AACGCTTCACGAATTTGCGT

NC mimics

UUCUCCGAACGUGUCACGUTT

ACGUGACACGUUCGGAGAATT

miR-146a mimics

UGAGAACUGAAUUCCAUGGGUU

CCCAUGGAAUUCAGUUCUCAUU

NC inhibitor

UUCUCCGAACGUGUCACGUTT

 

miR-146a inhibitor

AACCCAUGGAAUUCAGUUCUCA

 
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