Figure 1
From: AFF1 and AFF4 differentially regulate the osteogenic differentiation of human MSCs
Depletion of AFF1 improves osteogenic differentiation. (a) Real-time RT-PCR shows successful knockdown of AFF1. n=3. ***P<0.001. (b) Western blot analysis of AFF1. (c) Depletion of AFF1 inhibits the proliferation of MSCs. n=5. *P<0.05 and **P<0.01. (d) Representative images of alkaline phosphatase (ALP) staining. Depletion of AFF1 leads to more intense staining. (e) Quantitative analyses of ALP activity. n=5. *P<0.05. (f) Representative images of Alizarin red S (ARS) staining of MSCs. (g) Quantitative analyses of calcium mineralization. n=5. *P<0.05. (h–j) Real-time RT-PCR reveals elevated mRNA expression of RUNX2, SP7 and BGLAP. n=3. *P<0.05 and **P<0.01.
