Figure 4
From: AFF1 and AFF4 differentially regulate the osteogenic differentiation of human MSCs
Overexpression of AFF4 enhances osteogenic differentiation. (a) Real-time RT-PCR shows that the mRNA level of AFF4 is significantly increased. n=3. ***P<0.001. (b) Western blot analysis of AFF4. (c) Representative image of ALP staining. Overexpression of AFF4 leads to more intense staining. (d) Quantitative analyses of ALP activity. n=5. *P<0.05. (e) Representative images of ARS staining of MSCs. (f) Quantitative analyses of calcium mineralization. n=5. *P<0.001. (g–i) Real-time RT-PCR reveals increased mRNA expression of SP7 and BGLAP. n=3. *P<0.05 and **P<0.01.
