Figure 5
From: AFF1 and AFF4 differentially regulate the osteogenic differentiation of human MSCs
AFF1 and AFF4 differentially regulate bone formation in vivo. (a) Representative images of H&E staining of ectopic bone formation at 3 weeks. There is very little newly generated bone, while many β-TCP particles (the white bubble-like spots) remain. (b) Quantitative analyses of bone volume versus total tissue volume (BV/TV) at 3 weeks. n=5. *P<0.05 and **P<0.01. (c) Representative images of H&E staining at 6 weeks. Cells overexpressing HA-AFF1 form less bone tissue, while those overexpressing HA-AFF4 exhibit more bone formation. (d) BV/TV at 6 weeks. n=5. *P<0.05.
