Figure 6
From: AFF1 and AFF4 differentially regulate the osteogenic differentiation of human MSCs
AFF1 controls DKK1 transcription. (a) Real-time RT-PCR shows altered expression of DKK1. n=3. ***P<0.001. (b) Western blot analysis of DKK1. (c) A ChIP assay for AFF1 shows that it binds to the promoter region of DKK1. n=4. ***P<0.001. (d) Representative images of ALP staining. (e) Quantitative analyses of ALP activity. n=5. *P<0.05, **P<0.01 and ***P<0.001. (f, g) Real-time RT-PCR of SP7 and BGLAP. n=3. **P<0.01 and ***P<0.001.
