Figure 3
ASC-paracrine angiogenesis is modulated by Wnt regulators in vitro. (a) Formation of vessel-like structures in ASC-EC co-cultured 3D gels treated with DKK1 and LiCl by enhanced inverted microscopy. The left three groups show 7 days of treatments and the right three groups show 14 days of treatments. ASCs are GFP-positive and ECs are RFP-positive. The images shown are representative of four different experiments (n=4). (b) Formation of vessel-like structures in ASC-EC co-cultured 3D gels treated with DKK1 and LiCl imaged and reconstructed by modified CLSM. The left three groups show 7 days of treatments and the right three groups show 14 days of treatments. ASCs are GFP-positive and ECs are RFP-positive. The images shown are representative of four different experiments (n=4).
