Figure 3
From: Caspase-8 deficiency facilitates cellular transformation in vitro
Comparison of the rates of transformation of caspase-8+/+ and caspase-8−/− mouse embryonic fibroblasts (MEFs) in culture by a test of soft agar colony formation. Aliquots of the indicated numbers (‘n’) of independent MEF strains were examined. (a) Total colony size is plotted as a function of the passage number for each of the MEF strains (broken gray lines). Mean values±S.D. (solid lines) are also shown. (b, c) Typical microscopic appearance, 21 days after plating, of representative fields of the agar layer inoculated with WT and KO MEFs, or, for comparison, with cells of the MCF-7 breast carcinoma line
