Figure 1
From: L-selectin and SDF-1 enhance the migration of mouse and human cardiac mesoangioblasts
Induction of cardiac mesoangioblast transmigration by cytokines in vitro. (a) Mouse cardiac mesoangioblasts or fibroblasts were plated on endothelium-coated Transwell filters and induced to migrate for 6 h in presence of cardiomyocytes (CMCs) or different cytokines (30 ng/ml FGF; 50 ng/ml SDF-1; 80 ng/ml MCP-1; 30 ng/ml TNF-α; 10 ng/ml AMD3100, CXCR4 antagonist). A representative out of five independent experiments run in duplicate is shown (left; *α<0.02). A representative image of the transmigrated cardiac mesoangioblasts in the presence of CMCs, SDF-1 or MCP-1 is also shown. Bar, 30 μm. (b) Supernatants from fibroblasts or CMCs were collected after 4 days, and different cytokines were detected using the mouse multi-cytokine detection system. One out of three experiments is shown (*α<0.025)
